Gene Center Munich
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Technologies

Fly food and strain collection

We keep a large collection of fly strains (4,000 lines) and carry out genome-wide RNAi screens (10,000 VDRC lines); therefore our fly food needs are considerable. We have a setup for large-scale food preparation that includes a 150 l steam kettle with built-in stirrer, a peristaltic pump, and a robotic food dispenser. With this setup, Dagmar Schmid and Ruzica Barisic produce our weekly fly food supply in a single day; they also maintain our strain collection.

BioImaging

Bio-imaging unites the power of microscopy, biology, biophysics, biochemistry and advanced computational methods to study how genes and proteins function in living cells and organisms. Our Bio-Imaging Facility, established and managed by Christophe Jung provides access to high grade fluorescence research microscopes to observe and analyze fixed samples as well as live organisms. The facility holds a variety of microscopes and image processing tools, including a confocal microscope (inverted Zeiss LSM710), an epifluorescence microscope with Single Molecule detection capabilities (inverted Leica DM6000), a stereomicroscope (LEICA M205), as well as various software platforms for 3D volume rendering and image analysis (such as Metamorph, Imaris and Definiens Developer) and the Huygens deconvolution software.

Robotic high-throughput facility

Our robotic high-throughput facility provides state-of-the-art instrumentation and expertise to advance industrial-type research in academia. Run by Peter Bandilla and Christophe Jung, the facility is equipped with two independent high-end robotic systems. Each of them consists of two inter-connected flexible liquid handling workstations for multi- and single-channel pipetting, which are integrated with additional instruments for sample management, plate storage and handling. In addition, the facility houses detection and analysis systems, which permit assay read-out and quality control. Through this integrated design, high-throughput projects can be carried from assay development, to assay transfer, automation, validation and, finally, screening. A wide range of screening procedures can be set up in prokaryotic and eukaryotic cell systems, including protein expression studies and cell-based assays. Pipetting-intense lab routines, such as molecular biology protocols, can be handled by our set-up.