Arnold Lab - Proteomics
Collaborative Research Projects
We perform comprehensive proteomic analyses in a number of collaborative projects, covering a broad spectrum of biological aspects:
Spermatogonial stem cells (SSCs) are crucial for spermatogenesis in men and require a special milieu referred to as SSC niche. Via protein secretion, cells of the wall of seminiferous tubules, the so-called human testicular peritubular cells (HTPCs), are supposed to contribute to the SSC niche. Therefore, in collaboration with Prof. Artur Mayerhofer (Department for Cell Biology, LMU), we analyze the HTPC proteome as well as their secretome (Flenkenthaler et al., 2014). Furthermore, we study the influence of cellular ageing on the HTPC proteome and secretome, which is supposed to be an important factor for the decrease of reproductive function in men with advancing age.
Together with Prof. Christian Laforsch (Department of Biology, University Bayreuth), we analyze the molecular basis of phenotypic plasticity in the model organism Daphnia (Trotter et al., Otte et al., Fröhlich et al.). The Daphnia genome, published 2011, contains the so far highest number of genes, among them many with no functional homologues in other species. These genes are supposed to be responsible for the excellent ability of Daphnia to adapt to environmental stress conditions. In addition, a project investigating the influence of microplastic uptake on the proteome of Daphnia was recently started.
Due to chemoresistance formation, treatment options for various cancer types are limited. In a collaborative project with Dr. Andreas Roidl and Prof. Ernst Wagner (Department of Pharmacy, LMU), we monitor the development of chemoresistance in breast cancer cells at the proteome level. In a further collaborative research project with Prof. Angelika Vollmar (Department of Pharmacy, LMU) and Prof. Johanna Pachmayr (Paracelsus Medical University, Salzburg), we characterize sorafenib-resistant hepatocellular carcinoma cells at the proteome level, in order to decipher molecular mechanisms underlying sorafenib-resistance. In addition, we investigate the inhibition of Cdk5 as an innovative combination treatment to improve sorafenib responsiveness.
Platelets play a key role in haemostasis and various diseases including arterial thrombosis. Glycoprotein (GP) VI mediates adhesion to collagen structures exposed at sites of vascular injury and subsequent platelet activation. Together with Prof. Christian Schulz (Department of Internal Medicine I, LMU), we determined the effects of specific activation of GPVI on the human platelet proteome (Schulz et al., 2010).