Structural Biology: Ribosome
Unraveling the mechanisms of CAT tailing cycle
Beckmann group in collaboration with Inada lab unveils the molecular basis of eIF5A-dependent CAT tailing in eukaryotic ribosome-associated quality control.
Ribosome-associated quality control (RQC) is a conserved process degrading potentially toxic truncated nascent peptides whose malfunction underlies neurodegeneration and proteostasis decline in aging. During RQC, dissociation of stalled ribosomes is followed by elongation of the nascent peptide with alanine and threonine residues, driven by Rqc2 independently of mRNA, the small ribosomal subunit and guanosine triphosphate (GTP)-hydrolyzing factors. The resulting CAT tails (carboxy-terminal tails) and ubiquitination by Ltn1 mark nascent peptides for proteasomal degradation. Here we present ten cryogenic electron microscopy (cryo-EM) structures, revealing the mechanistic basis of individual steps of the CAT tailing cycle covering initiation, decoding, peptidyl transfer, and tRNA translocation. We discovered eIF5A as a crucial eukaryotic RQC factor enabling peptidyl transfer. Moreover, we observed dynamic behavior of RQC factors and tRNAs allowing for processivity of the CAT tailing cycle without additional energy input. Together, these results elucidate key differences as well as common principles between CAT tailing and canonical translation.
Cryo-EM structural intermediates of the CAT tailing cycle: Ensemble of cryo-EM density maps arranged in the most plausible order of steps during the CAT tailing cycle. Individual components in each intermediate state are highlighted as segregated colored maps. Source: Mol. Cell 2023
Molecular basis of eIF5A-dependent CAT tailing in eukaryotic ribosome-associated quality control
Tesina R, Ebine S, Buschauer R, Thoms M, Matsuo Y, Inada T, Beckmann R.
Mol. Cell 2023 Feb 16; https://doi.org/10.1016/j.molcel.2023.01.020