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Flow Cytometry Analysis

General Information

Flow cytometry is a tool in biotechnology that simultaneously measures and analyzes physical and chemical characteristics of single cells as they flow in a fluid stream through a beam of light. Particle’s relative size, relative granularity, internal complexity, and relative fluorescence intensity can be measured with flow cytometry. Multiparametric phenotypic analysis by flow cytometry allows researchers to study numerous applications in various aspects of biological research, immunology and pathology.

The BD LSRFortessa with four lasers, (405, 488, 561 and 640 nm) is able to detect up to 16 colors simultaneously and delivers the optimal sensitivity and resolution required for multicolor applications. In addition, the facility is equipped with the High Throughput Sampler (HTS) which provides an automated acquisition of samples from a 96-well plate.

  • Please download the Analysis Request, fill out and send to us.
  • Please download the PDF for our default Fortessa Configuration.

Analyzing involves using a flow cytometer to interrogate cells stained with fluorescent markers and classifying them into groups. Cells pass through the instrument once and go straight to a waste container. There is no cell recovery. As single-cell suspensions are required for all flow cytometry assays, peripheral blood cells or cells that grow in suspension are well suited for analysis by the flow cytometry. Adherent cell lines, solid tissue samples, and tumors need proper processing before they can be analysed.

If you need more information such as which combinations of dyes or fluorescent proteins are possible to use, please contact us (tafrishi@genzentrum.lmu.de).

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