Cryo-EM Facility - Gene Center LMU
The Gene Center Cryo-EM core facility, funded by the LMU together with the DFG (Deutsche Forschungsgemeinschaft) in the context of the research network CRC646, was established in 2007 by Prof. Roland Beckmann and is located at the ICEM.
Cryo-EM sample preparation and analysis has since then been performed, using three electron microscopes including one of the first high-end Titan Krios microscopes, which is equipped with a Bioquantum energy filter and K2 direct detector.
In 2017, as a result of a joint effort by Profs. Karl-Peter Hopfner and Roland Beckmann, funding by the LMU and the DFG allowed to establish a second Titan Krios (G3), which is equipped with a phase plate and a Falcon 4i detector and Selectris X energy filter combination.
As a core facility of the Gene Center we are happy to support your cryo-EM projects.
The state-of-the-art setup permits (1) sample/grid preparation for negative stain, (2) sample/grid vitrification for cryo-EM, (3) low resolution cryo-EM screening as well as (4) large-scale data collection for single particle reconstructions at near-atomic resolution.
We welcome users from the LMU Gene Center, the Faculty of Chemistry and Pharmacy and other academic institutions of the LMU, as our capacities allow.
| Location | Operating hours | Contact |
|---|---|---|
| ICEM LMU Würmtalstr. 201 81377 München |
8:30 - 17:30 Certain instruments are open to trained users 24/7 |
berninghausen@genzentrum.lmu.de |
