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Instruments

Titan Krios

The Titan Krios is a 300 KV transmission electron microscope ideal for high resolution cryoelectron microscopy or cryoelectron tomography.

titan

Key features:

  • 80-300kV tunable, FEG
  • 80-300kV tunable, FEG
  • Cryo-autoloader sample stage (up to 12 grids/load)
  • 4K FalconII for for high resolution cryoEM imaging
  • Double-tilt cryoET
  • Automated acquisition of large data set

Titan Krios G3

The Titan Krios G3 is a 300 KV transmission electron microscope ideal for high resolution cryoelectron microscopy or cryoelectron tomography.

titan krios g3

Key features:

  • 80-300kV tunable, FEG
  • Cryo-autoloader sample stage (up to 12 grids/load)
  • 4K FalconIII for for high resolution cryoEM imaging
  • Gatan K2 camera for high resolution cryoEM imaging of smal protein complexes
  • Double-tilt cryoET
  • Automated acquisition of large data sets

Morgagni

The Morgagni is an 100 KV transmission electron microscope ideal for routine sample screening and high contrast visualization of plastic sections.

morgagni

Key features:

  • 100kV
  • Tungsten filament
  • computer-controlled interface
  • very short on-time

Images captured from the Morgagni can be recorded on a side-mounted SIS Megaview 1K CCD camera

Tecnai G2 Spirit

The SPIRIT is an 120 KV transmission electron microscope ideal for cryoelectron microscopy or cryoelectron tomography.

spirit

Key features:

  • 120kV
  • LaB6 filament
  • computer-controlled cryostage
  • suitable for both cryoEM and cryoET

Images captured from the Spirit can be recorded on ultrafast CMOS based TVIPS XF216 CCD camera. The image processing programs installed are EMMENU4 and FEI Xplore3D Tomography Package.

Vitrobot Marc IV

The Vitrobot™ (Vitrification Robot) is a fully PC-controlled device for vitrification (rapid cooling) of aqueous samples.

vitrobot

  • The process of plunging, blotting, and vitrification is fully automated upon placing a vial into the chamber and setting up the ethane holder.
  • Operation is strictly controlled by dedicated hardware and software, implying that parameters and results are reproducible.
  • This enables a high throughput of vitrified samples, with an easy and straightforward control of the vitrification process.
  • The parameters to be influenced are: temperature, humidity, the number of blots, and a number of critical time settings.
  • In addition to this automated plunger, our lab also has a uniquely crafted manual plunger for anyone who would rather perform this process manually.

Plasma Cleaner and Glow Discharger

plasma cleanerPlasma cleaner and Glow discharger to render hydrophobic carbon hydrophilic.