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Beckmann Lab - Cryo-EM and Single Particle Analysis

Research Topics

    • Cryo-Electron Microscopy
    • Quality Control and mRNA Surveillance
    • Protein Targeting and Translocation
    • Ribosome Biogenesis

The ribosome serves, apart from its function in translation, as platform for various regulatory processes which are at the center of our research. Here, we focus on understanding molecular mechanisms of gene expression regulation, mRNA surveillance and ribosome quality control, co-translational targeting and translocation of proteins to and across a target membrane and assembly of the 40S and 60S ribosomal subunits using cryo-EM. Read more...

Selected Publications

The cryo-EM structure of a ribosome-Ski2-Ski3-Ski8 helicase complex.
Schmidt C, Kowalinski E, Shanmuganathan V, Defenouillère Q, Braunger K, Heuer A, Pech M, Namane A, Berninghausen O, Fromont-Racine M, Jacquier A, Conti E, Becker T, Beckmann R.
Science. 2016 Dec 16;354(6318):1431-1433. PubMed

Architecture of the 90S Pre-ribosome: A Structural View on the Birth of the Eukaryotic Ribosome.
Kornprobst M, Turk M, Kellner N, Cheng J, Flemming D, Koš-Braun I, Koš M, Thoms M, Berninghausen O, Beckmann R, Hurt E.
Cell. 2016 Jul 14;166(2):380-93. doi: 10.1016/j.cell.2016.06.014. PubMed

The force-sensing peptide VemP employs extreme compaction and secondary structure formation to induce ribosomal stalling.
Su T, Cheng J, Sohmen D, Hedman R, Berninghausen O, von Heijne G, Wilson DN, Beckmann R.
Elife. 2017 May 30;6. pii: e25642. doi: 10.7554/eLife.25642. PubMed

Structures of the Sec61 complex engaged in nascent peptide translocation or membrane insertion.
Gogala M, Becker T, Beatrix B, Armache JP, Barrio-Garcia C, Berninghausen O, Beckmann R.
Nature. 2014 Feb 6;506(7486):107-10. doi: 10.1038/nature12950. PubMed

More publications

News

14.12.2017 - New Publication

Visualizing the Assembly Pathway of Nucleolar Pre-60S Ribosomes.

Using cryo-EM, we solved structures of early 60S biogenesis intermediates at 3.3 Å to 4.5 Å resolution, thereby providing insights into their sequential folding and assembly pathway. read more

20.11.2017 - New Publication

Cryo-EM structure of a late pre-40S ribosomal subunit from Saccharomyces cerevisiae.

We present the structure of a late pre-40S particle at 3.6 Å resolution, revealing in molecular detail how assembly factors regulate the timely folding of pre-18S rRNA. read more