Keppler Lab - Publications
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2022
LFA1 and ICAM1 are critical for fusion and spread of murine leukemia virus in vivo.
Engels R, Falk L, Albanese M, Keppler OT, Sewald X.
Cell Rep. 2022 Jan 18;38(3):110279. doi: 10.1016/j.celrep.2021.110279. PubMed
Evidence for increased SARS-CoV-2 susceptibility and COVID-19 severity related to pre-existing immunity to seasonal coronaviruses.
Wratil PR, Schmacke NA, Karakoc B, Dulovic A, Junker D, Becker M, Rothbauer U, Osterman A, Spaeth PM, Ruhle A, Gapp M, Schneider S, Muenchhoff M, Hellmuth JC, Scherer C, Mayerle J, Reincke M, Behr J, Kääb S, Zwissler B, von Bergwelt-Baildon M, Eberle J, Kaderali L, Schneiderhan-Marra N, Hornung V, Keppler OT.
Cell Rep. 2021 Dec 28;37(13):110169. doi: 10.1016/j.celrep.2021.110169. Epub 2021 Dec 7. PubMed
MicroRNAs are minor constituents of extracellular vesicles that are rarely delivered to target cells.
Albanese M, Chen YA, Hüls C, Gärtner K, Tagawa T, Mejias-Perez E, Keppler OT, Göbel C, Zeidler R, Shein M, Schütz AK, Hammerschmidt W.
PLoS Genet. 2021 Dec 6;17(12):e1009951. doi: 10.1371/journal.pgen.1009951. eCollection 2021 Dec. PubMed
SARS-CoV-2 variants of concern display enhanced intrinsic pathogenic properties and expanded organ tropism in mouse models.
Stolp B, Stern M, Ambiel I, Hofmann K, Morath K, Gallucci L, Cortese M, Bartenschlager R, Ruggieri A, Graw F, Rudelius M, Keppler OT, Fackler OT.
Cell Rep. 2022 Feb 15;38(7):110387. doi: 10.1016/j.celrep.2022.110387. Epub 2022 Jan 28. PubMed
Three exposures to the spike protein of SARS-CoV-2 by either infection or vaccination elicit superior neutralizing immunity to all variants of concern.
Wratil PR, Stern M, Priller A, Willmann A, Almanzar G, Vogel E, Feuerherd M, Cheng CC, Yazici S, Christa C, Jeske S, Lupoli G, Vogt T, Albanese M, Mejías-Pérez E, Bauernfried S, Graf N, Mijocevic H, Vu M, Tinnefeld K, Wettengel J, Hoffmann D, Muenchhoff M, Daechert C, Mairhofer H, Krebs S, Fingerle V, Graf A, Steininger P, Blum H, Hornung V, Liebl B, Überla K, Prelog M, Knolle P, Keppler OT, Protzer U. Nat Med. 2022 Jan 28. doi: 10.1038/s41591-022-01715-4. PubMed
Rapid and sensitive identification of omicron by variant-specific PCR and nanopore sequencing: paradigm for diagnostics of emerging SARS-CoV-2 variants.
Dächert C, Muenchhoff M, Graf A, Autenrieth H, Bender S, Mairhofer H, Wratil PR, Thieme S, Krebs S, Grzimek-Koschewa N, Blum H, Keppler OT.
Med Microbiol Immunol. 2022 Jan 21:1-7. doi: 10.1007/s00430-022-00728-7. PubMed
Rapid, efficient and activation-neutral gene editing of polyclonal primary human resting CD4+ T cells allows complex functional analyses.
Albanese M, Ruhle A, Mittermaier J, Mejías-Pérez E, Gapp M, Linder A, Schmacke NA, Hofmann K, Hennrich AA, Levy DN, Humpe A, Conzelmann KK, Hornung V, Fackler OT, Keppler OT.
Nat Methods. 2022 Jan;19(1):81-89. doi: 10.1038/s41592-021-01328-8. Epub 2021 Dec 23. PubMed
2021
Picomolar inhibition of SARS-CoV-2 variants of concern by an engineered ACE2-IgG4-Fc fusion protein.
Svilenov HL, Sacherl J, Reiter A, Wolff LS, Cheng CC, Stern M, Grass V, Feuerherd M, Wachs FP, Simonavicius N, Pippig S, Wolschin F, Keppler OT, Buchner J, Brockmeyer C, Protzer U.
Antiviral Res. 2021 Dec;196:105197. doi: 10.1016/j.antiviral.2021.105197. Epub 2021 Nov 10. PubMed
Genomic epidemiology reveals multiple introductions of SARS-CoV-2 followed by community and nosocomial spread, Germany, February to May 2020.
Muenchhoff M, Graf A, Krebs S, Quartucci C, Hasmann S, Hellmuth JC, Scherer C, Osterman A, Boehm S, Mandel C, Becker-Pennrich AS, Zoller M, Stubbe HC, Munker S, Munker D, Milger K, Gapp M, Schneider S, Ruhle A, Jocham L, Nicolai L, Pekayvaz K, Weinberger T, Mairhofer H, Khatamzas E, Hofmann K, Spaeth PM, Bender S, Kääb S, Zwissler B, Mayerle J, Behr J, von Bergwelt-Baildon M, Reincke M, Grabein B, Hinske CL, Blum H, Keppler OT.
Euro Surveill. 2021 Oct;26(43):2002066. doi: 10.2807/1560-7917.ES.2021.26.43.2002066. PubMed
Impaired function and delayed regeneration of dendritic cells in COVID-19.
Winheim E, Rinke L, Lutz K, Reischer A, Leutbecher A, Wolfram L, Rausch L, Kranich J, Wratil PR, Huber JE, Baumjohann D, Rothenfusser S, Schubert B, Hilgendorff A, Hellmuth JC, Scherer C, Muenchhoff M, von Bergwelt-Baildon M, Stark K, Straub T, Brocker T, Keppler OT, Subklewe M, Krug AB.
PLoS Pathog. 2021 Oct 6;17(10):e1009742. doi: 10.1371/journal.ppat.1009742. eCollection 2021 Oct. PubMed
Comparison of four commercial, automated antigen tests to detect SARS-CoV-2 variants of concern.
Osterman A, Iglhaut M, Lehner A, Späth P, Stern M, Autenrieth H, Muenchhoff M, Graf A, Krebs S, Blum H, Baiker A, Grzimek-Koschewa N, Protzer U, Kaderali L, Baldauf HM, Keppler OT.
Med Microbiol Immunol. 2021 Dec;210(5-6):263-275. doi: 10.1007/s00430-021-00719-0. Epub 2021 Aug 20. PubMed
ADP Ribosylation Factor 6 Promotes Contraction and Proliferation, Suppresses Apoptosis and Is Specifically Inhibited by NAV2729 in Prostate Stromal Cells.
Wang R, Schneider S, Keppler OT, Li B, Rutz B, Ciotkowska A, Stief CG, Hennenberg M.
Mol Pharmacol. 2021 Oct;100(4):356-371. doi: 10.1124/molpharm.121.000304. Epub 2021 Aug 4. PubMed
Spatially resolved qualified sewage spot sampling to track SARS-CoV-2 dynamics in Munich - One year of experience.
Rubio-Acero R, Beyerl J, Muenchhoff M, Roth MS, Castelletti N, Paunovic I, Radon K, Springer B, Nagel C, Boehm B, Böhmer MM, Graf A, Blum H, Krebs S, Keppler OT, Osterman A, Khan ZN, Hoelscher M, Wieser A; KoCo19-Study Group.
Sci Total Environ. 2021 Nov 25;797:149031. doi: 10.1016/j.scitotenv.2021.149031. Epub 2021 Jul 21. PubMed
Letermovir in lung transplant recipients with cytomegalovirus infection: A retrospective observational study.
Veit T, Munker D, Barton J, Milger K, Kauke T, Meiser B, Michel S, Zoller M, Nitschko H, Keppler OT, Behr J, Kneidinger N.
Am J Transplant. 2021 Oct;21(10):3449-3455. doi: 10.1111/ajt.16718. Epub 2021 Jul 3. PubMed
Thermal Inactivation of Carcasses of Mice and Rabbits Infected with Pathogens of Risk Groups Two to Four.
Baldauf HM, Weingartner S, Hofmann K, Mitteregger-Kretzschmar G, Popper B, Bönisch MP, Keppler OT.
J Am Assoc Lab Anim Sci. 2021 Jul 1;60(4):451-461. doi: 10.30802/AALAS-JAALAS-20-000097. Epub 2021 May 25. PubMed
Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infection.
Akidil E, Albanese M, Buschle A, Ruhle A, Pich D, Keppler OT, Hammerschmidt W.
PLoS Pathog. 2021 Apr 15;17(4):e1009117. doi: 10.1371/journal.ppat.1009117. eCollection 2021 Apr. PubMed
Dynamics of SARS-CoV-2 shedding in the respiratory tract depends on the severity of disease in COVID-19 patients.
Munker D, Osterman A, Stubbe H, Muenchhoff M, Veit T, Weinberger T, Barnikel M, Mumm JN, Milger K, Khatamzas E, Klauss S, Scherer C, Hellmuth JC, Giessen-Jung C, Zoller M, Herold T, Stecher S, de Toni EN, Schulz C, Kneidinger N, Keppler OT, Behr J, Mayerle J, Munker S.
Eur Respir J. 2021 Jul 20;58(1):2002724. doi: 10.1183/13993003.02724-2020. Print 2021 Jul. PubMed
Evaluation of two rapid antigen tests to detect SARS-CoV-2 in a hospital setting.
Osterman A, Baldauf HM, Eletreby M, Wettengel JM, Afridi SQ, Fuchs T, Holzmann E, Maier A, Döring J, Grzimek-Koschewa N, Muenchhoff M, Protzer U, Kaderali L, Keppler OT.
Med Microbiol Immunol. 2021 Feb;210(1):65-72. doi: 10.1007/s00430-020-00698-8. Epub 2021 Jan 16. PubMed
Prospective Longitudinal Serosurvey of Healthcare Workers in the First Wave of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Pandemic in a Quaternary Care Hospital in Munich, Germany.
Weinberger T, Steffen J, Osterman A, Mueller TT, Muenchhoff M, Wratil PR, Graf A, Krebs S, Quartucci C, Spaeth PM, Grabein B, Adorjan K, Blum H, Keppler OT, Klein M.
Clin Infect Dis. 2021 Nov 2;73(9):e3055-e3065. doi: 10.1093/cid/ciaa1935. PMID: 3338875 PubMed
2020
Complex human adenoid tissue-based ex vivo culture systems reveal anti-inflammatory drug effects on germinal center T and B cells.
Schmidt A, Huber JE, Sercan Alp Ö, Gürkov R, Reichel CA, Herrmann M, Keppler OT, Leeuw T, Baumjohann D.
EBioMedicine. 2020 Feb 27;53:102684. doi: 10.1016/j.ebiom.2020.102684. PubMed
Human FCHO1 deficiency reveals role for clathrin-mediated endocytosis in development and function of T cells.
Łyszkiewicz M, Ziętara N, Frey L, Pannicke U, Stern M, Liu Y, Fan Y, Puchałka J, Hollizeck S, Somekh I, Rohlfs M, Yilmaz T, Ünal E, Karakukcu M, Patiroğlu T, Kellerer C, Karasu E, Sykora KW, Lev A, Simon A, Somech R, Roesler J, Hoenig M, Keppler OT, Schwarz K, Klein C.
Nat Commun. 2020 Feb 25;11(1):1031. doi: 10.1038/s41467-020-14809-9. PubMed
Comparative multi-assay evaluation of Determine™ HIV-1/2 Ag/Ab Combo rapid diagnostic tests in acute and chronic HIV infection.
Wratil PR, Rabenau HF, Eberle J, Stern M, Münchhoff M, Friedrichs I, Stürmer M, Berger A, Kuttner-May S, Münstermann D, Lucht A, Meixenberger K, Bannert N, Keppler OT.
Med Microbiol Immunol. 2020 Feb 8. doi: 10.1007/s00430-019-00655-0. PubMed
SERINC5 Is an Unconventional HIV Restriction Factor That Is Upregulated during Myeloid Cell Differentiation.
Zutz A, Schölz C, Schneider S, Pierini V, Münchhoff M, Sutter K, Wittmann G, Dittmer U, Draenert R, Bogner JR, Fackler OT, Keppler OT.
J Innate Immun. 2020 Jan 14:1-11. doi: 10.1159/000504888. PubMed
2019
Selective inactivation of hypomethylating agents by SAMHD1 provides a rationale for therapeutic stratification in AML. Oellerich T+, Schneider C+, Thomas D, Knecht KM, Buzovetsky O, Kaderali L, Schliemann C, Bohnenberger H, Angenendt L, Hartmann W, Wardelmann E, Rothenburger T, Mohr S, Scheich S, Comoglio F, Wilke A, Ströbel P, Serve H, Michaelis M, Ferreirós N, Geisslinger G, Xiong Y, Keppler OT*, Cinatl J Jr*.
Nat Commun. 2019 Aug 2;10(1):3475. doi: 10.1038/s41467-019-11413-4. PubMed
Differential Pathogen-Specific Immune Reconstitution in Antiretroviral Therapy-Treated Human Immunodeficiency Virus-Infected Children.
Muenchhoff M, Adland E, Roider J, Kløverpris H, Leslie A, Boehm S, Keppler OT, Ndung'u T, Goulder PJR.
J Infect Dis. 2019 Jan 8. doi: 10.1093/infdis/jiy668. PubMed
The antiviral activity of rodent and lagomorph SERINC3 and SERINC5 is counteracted by known viral antagonists.
de Sousa-Pereira P, Abrantes J, Bauernfried S, Pierini V, Esteves PJ, Keppler OT, Pizzato M, Hornung V, Fackler OT, Baldauf HM.
J Gen Virol. 2018 Dec 19. doi: 10.1099/jgv.0.001201. PubMed
The structural basis for cancer drug interactions with the catalytic and allosteric sites of SAMHD1.
Knecht KM, Buzovetsky O, Schneider C, Thomas D, Srikanth V, Kaderali L, Tofoleanu F, Reiss K, Ferreirós N, Geisslinger G, Batista VS, Ji X, Cinatl J Jr, Keppler OT, Xiong Y.
PNAS. 2018 Oct 23;115(43):E10022-E10031. doi: 10.1073/pnas.1805593115. PubMed
Post-translational Modification-Based Regulation of HIV Replication.
Chen L, Keppler OT, Schölz C.
Front Microbiol. 2018 Sep 11;9:2131. doi: 10.3389/fmicb.2018.02131. eCollection 2018. Review. PubMed
The wide utility of rabbits as models of human diseases.
Esteves PJ, Abrantes J, Baldauf HM, BenMohamed L, Chen Y, Christensen N, González-Gallego J, Giacani L, Hu J, Kaplan G, Keppler OT, Knight KL, Kong XP, Lanning DK, Le Pendu J, de Matos AL, Liu J, Liu S, Lopes AM, Lu S, Lukehart S, Manabe YC, Neves F, McFadden G, Pan R, Peng X, de Sousa-Pereira P, Pinheiro A, Rahman M, Ruvoën-Clouet N, Subbian S, Tuñón MJ, van der Loo W, Vaine M, Via LE, Wang S, Mage R.
Exp Mol Med. 2018 May 22;50(5):66. doi: 10.1038/s12276-018-0094-1. Review. PubMed
2017
Corrigendum: SAMHD1 is a biomarker for cytarabine response and a therapeutic target in acute myeloid leukemia.
Schneider C, Oellerich T, Baldauf HM, Schwarz SM, Thomas D, Flick R, Bohnenberger H, Kaderali L, Stegmann L, Cremer A, Martin M, Lohmeyer J, Michaelis M, Hornung V, Schliemann C, Berdel WE, Keppler OT, Hartmann W, Wardelmann E, Comoglio F, Hansmann ML, Yakunin AF, Geisslinger G, et al.
Nat Med. 2017 Jun 6;23(6):788. doi: 10.1038/nm0617-788a. No abstract available. PubMed
Retrospective analysis of clinical and virological parameters of influenza cases at four university hospitals in Germany, 2015.
Heyd R, Eis-Hübinger AM, Berger A, Bierbaum S, Pietzonka S, Wenzel JJ, Huzly D, Keppler OT, Panning M.
Infection. 2017 Jun;45(3):349-354. doi: 10.1007/s15010-017-1008-1. Epub 2017 Mar 18. PubMed
Elevated prevalence of multidrug-resistant gram-negative organisms in HIV positive men.
Reinheimer C, Keppler OT, Stephan C, Wichelhaus TA, Friedrichs I, Kempf VA.
BMC Infect Dis. 2017 Mar 13;17(1):206. doi: 10.1186/s12879-017-2286-z. PubMed
HCV RNA Testing of Plasma Samples from Cornea Donors: Suitability of Plasma Samples Stored at 4 °C for up to 8 Days. Berger A, Salla S, Keppler OT, Rabenau HF.
Transfus Med Hemother. 2017 Jan;44(1):39-44. doi: 10.1159/000449207. Epub 2016 Nov 4. PubMed
Vpx overcomes a SAMHD1-independent block to HIV reverse transcription that is specific to resting CD4 T cells.
Baldauf HM, Stegmann L, Schwarz SM, Ambiel I, Trotard M, Martin M, Burggraf M, Lenzi GM, Lejk H, Pan X, Fregoso OI, Lim ES, Abraham L, Nguyen LA, Rutsch F, König R, Kim B, Emerman M, Fackler OT, Keppler OT.
Proc Natl Acad Sci U S A. 2017 Mar 7;114(10):2729-2734. doi: 10.1073https://www.ncbi.nlm.nih.gov/pnas.1613635114. Epub 2017 Feb 22. PubMed
2016
SAMHD1 is a biomarker for cytarabine response and a therapeutic target in acute myeloid leukemia.
Schneider C, Oellerich T, Baldauf HM, Schwarz SM, Thomas D, Flick R, Bohnenberger H, Kaderali L, Stegmann L, Cremer A, Martin M, Lohmeyer J, Michaelis M, Hornung V, Schliemann C, Berdel WE, Hartmann W, Keppler OT, Wardelmann E, Comoglio F, Hansmann ML, Yakunin AF, Geisslinger G, et al. Nat Med. 2017 Feb;23(2):250-255. doi: 10.1038/nm.4255. Epub 2016 Dec 19. PubMed
Complex Interplay between HIV-1 Capsid and MX2-Independent Alpha Interferon-Induced Antiviral Factors.
Bulli L, Apolonia L, Kutzner J, Pollpeter D, Goujon C, Herold N, Schwarz SM, Giernat Y, Keppler OT, Malim MH, Schaller T.
J Virol. 2016 Jul 27;90(16):7469-80. doi: 10.1128/JVI.00458-16. Print 2016 Aug 15. PubMed
HIV-1 Vpu Antagonizes CD317/Tetherin by Adaptor Protein-1-Mediated Exclusion from Virus Assembly Sites.
Pujol FM, Laketa V, Schmidt F, Mukenhirn M, Müller B, Boulant S, Grimm D, Keppler OT, Fackler OT.
J Virol. 2016 Jul 11;90(15):6709-23. doi: 10.1128/JVI.00504-16. Print 2016 Aug 1. PubMed
Evolutionary study of leporid CD4 reveals a hotspot of genetic variability within the D2 domain.
de Sousa-Pereira P, Abrantes J, Baldauf HM, Keppler OT, Esteves PJ.
Immunogenetics. 2016 Jul;68(6-7):477-82. doi: 10.1007/s00251-016-0909-y. Epub 2016 Mar 15. PubMed
Prevalence of K65R in patients treated with tenofovir disoproxil fumarate: recommendations based on the Frankfurt HIV Cohort Study Resistance Database (FHCS-RD).
Reinheimer C, Wesner A, Keppler OT, Doerr HW, Herrmann E, Stürmer M, Stephan C.
Med Microbiol Immunol. 2016 Aug;205(4):315-20. doi: 10.1007/s00430-015-0448-4. Epub 2016 Jan 8. PubMed
2015
No SEVI-mediated enhancement of rectal HIV-1 transmission of HIV-1 in two humanized mouse cohorts.
Van Dis ES, Moore TC, Lavender KJ, Messer RJ, Keppler OT, Verheyen J, Dittmer U, Hasenkrug KJ.
Virology. 2016 Jan 15;488:88-95. doi: 10.1016/j.virol.2015.11.005. Epub 2015 Nov 21. PubMed
Mouse Siglec-1 Mediates trans-Infection of Surface-bound Murine Leukemia Virus in a Sialic Acid N-Acyl Side Chain-dependent Manner.
Erikson E, Wratil PR, Frank M, Ambiel I, Pahnke K, Pino M, Azadi P, Izquierdo-Useros N, Martinez-Picado J, Meier C, Schnaar RL, Crocker PR, Reutter W, Keppler OT.
J Biol Chem. 2015 Nov 6;290(45):27345-59. doi: 10.1074/jbc.M115.681338. Epub 2015 Sep 14. PubMed
Vpx mediated degradation of SAMHD1 has only a very limited effect on lentiviral transduction rate in ex vivo cultured HSPCs.
Li D, Schlaepfer E, Audigé A, Rochat MA, Ivic S, Knowlton CN, Kim B, Keppler OT, Speck RF.
Stem Cell Res. 2015 Sep;15(2):271-80. doi: 10.1016/j.scr.2015.06.012. Epub 2015 Jul 3. PubMed
Complement-Opsonized HIV-1 Overcomes Restriction in Dendritic Cells.
Posch W, Steger M, Knackmuss U, Blatzer M, Baldauf HM, Doppler W, White TE, Hörtnagl P, Diaz-Griffero F, Lass-Flörl C, Hackl H, Moris A, Keppler OT, Wilflingseder D.
PLoS Pathog. 2015 Jun 29;11(6):e1005005. doi: 10.1371/journal.ppat.1005005. eCollection 2015 Jun. PubMed
Management of Microbiological Samples in a Confirmed Case of Ebola Virus Disease: Constraints and Limitations.
Hogardt M, Wolf T, Kann G, Brodt HR, Brandt C, Keppler OT, Wicker S, Zacharowski K, Gottschalk R, Becker S, Kempf VA.
J Clin Microbiol. 2015 Nov;53(11):3396-400. doi: 10.1128/JCM.01142-15. Epub 2015 Jun 24. PubMed
HIV-1 immune activation induces Siglec-1 expression and enhances viral trans-infection in blood and tissue myeloid cells.
Pino M, Erkizia I, Benet S, Erikson E, Fernández-Figueras MT, Guerrero D, Dalmau J, Ouchi D, Rausell A, Ciuffi A, Keppler OT, Telenti A, Kräusslich HG, Martinez-Picado J, Izquierdo-Useros N.
Retrovirology. 2015 May 7;12:37. doi: 10.1186/s12977-015-0160-x. PubMed
Quantitation of endogenous nucleoside triphosphates and nucleosides in human cells by liquid chromatography tandem mass spectrometry.
Thomas D, Herold N, Keppler OT, Geisslinger G, Ferreirós N.
Anal Bioanal Chem. 2015 May;407(13):3693-704. doi: 10.1007/s00216-015-8588-3. Epub 2015 Mar 8. PubMed
SAMHD1's protein expression profile in humans.
Schmidt S, Schenkova K, Adam T, Erikson E, Lehmann-Koch J, Sertel S, Verhasselt B, Fackler OT, Lasitschka F, Keppler OT.
J Leukoc Biol. 2015 Jul;98(1):5-14. doi: 10.1189/jlb.4HI0714-338RR. Epub 2015 Feb 2. PubMed
2014
Severe Ebola virus disease with vascular leakage and multiorgan failure: treatment of a patient in intensive care.
Wolf T, Kann G, Becker S, Stephan C, Brodt HR, de Leuw P, Grünewald T, Vogl T, Kempf VA, Keppler OT, Zacharowski K.
Lancet. 2015 Apr 11;385(9976):1428-35. doi: 10.1016/S0140-6736(14)62384-9. Epub 2014 Dec 19. PubMed
HIV-1 Nef and Vpu are functionally redundant broad-spectrum modulators of cell surface receptors, including tetraspanins.
Haller C, Müller B, Fritz JV, Lamas-Murua M, Stolp B, Pujol FM, Keppler OT, Fackler OT.
J Virol. 2014 Dec;88(24):14241-57. doi: 10.1128/JVI.02333-14. Epub 2014 Oct 1. PubMed
Cell cycle control and HIV-1 susceptibility are linked by CDK6-dependent CDK2 phosphorylation of SAMHD1 in myeloid and lymphoid cells.
Pauls E, Ruiz A, Badia R, Permanyer M, Gubern A, Riveira-Muñoz E, Torres-Torronteras J, Alvarez M, Mothe B, Brander C, Crespo M, Menéndez-Arias L, Clotet B, Keppler OT, Martá R, Posas F, Ballana E, Esté JA.
J Immunol. 2014 Aug 15;193(4):1988-97. doi: 10.4049/jimmunol.1400873. Epub 2014 Jul 11. PubMed
Impact of raltegravir on HIV-1 RNA and DNA forms following initiation of antiretroviral therapy in treatment-naive patients.
Stephan C, Baldauf HM, Barry J, Giordano FA, Bartholomae CC, Haberl A, Bickel M, Schmidt M, Laufs S, Kaderali L, Keppler OT.
J Antimicrob Chemother. 2014 Oct;69(10):2809-18. doi: 10.1093/jac/dku213. Epub 2014 Jun 23. PubMed
Differential loss of humoral immunity against measles, mumps, rubella and varicella-zoster virus in children treated for cancer.
Bochennek K, Allwinn R, Langer R, Becker M, Keppler OT, Klingebiel T, Lehrnbecher T.
Vaccine. 2014 Jun 5;32(27):3357-61. doi: 10.1016/j.vaccine.2014.04.042. Epub 2014 Apr 29. PubMed
p21 regulates the HIV-1 restriction factor SAMHD1.
Pauls E, Ruiz A, Riveira-Muñoz E, Permanyer M, Badia R, Clotet B, Keppler OT, Ballana E, Este JA.
Proc Natl Acad Sci U S A. 2014 Apr 8;111(14):E1322-4. doi: 10.1073https://www.ncbi.nlm.nih.gov/pnas.1322059111. Epub 2014 Mar 7. No abstract available. PubMed
2013
MxB/Mx2: the latest piece in HIV's interferon puzzle.
Fackler OT, Keppler OT.
EMBO Rep. 2013 Dec;14(12):1028-9. doi: 10.1038/embor.2013.172. Epub 2013 Nov 1. No abstract available. PubMed
Restrictions to HIV-1 replication in resting CD4+ T lymphocytes.
Pan X, Baldauf HM, Keppler OT, Fackler OT.
Cell Res. 2013 Jul;23(7):876-85. doi: 10.1038/cr.2013.74. Epub 2013 Jun 4. Review. PubMed
Low vitamin D serum concentration is associated with high levels of hepatitis B virus replication in chronically infected patients.
Farnik H, Bojunga J, Berger A, Allwinn R, Waidmann O, Kronenberger B, Keppler OT, Zeuzem S, Sarrazin C, Lange CM.
Hepatology. 2013 Oct;58(4):1270-6. doi: 10.1002/hep.26488. Epub 2013 Aug 7. PubMed
2012
Siglec-1 is a novel dendritic cell receptor that mediates HIV-1 trans-infection through recognition of viral membrane gangliosides.
Izquierdo-Useros N, Lorizate M, Puertas MC, Rodriguez-Plata MT, Zangger N, Erikson E, Pino M, Erkizia I, Glass B, Clotet B, Keppler OT, Telenti A, Kräusslich HG, Martinez-Picado J.
PLoS Biol. 2012;10(12):e1001448. doi: 10.1371/journal.pbio.1001448. Epub 2012 Dec 18. PubMed
SAMHD1 restricts HIV-1 infection in resting CD4(+) T cells.
Baldauf HM, Pan X, Erikson E, Schmidt S, Daddacha W, Burggraf M, Schenkova K, Ambiel I, Wabnitz G, Gramberg T, Panitz S, Flory E, Landau NR, Sertel S, Rutsch F, Lasitschka F, Kim B, König R, Fackler OT, Keppler OT.
Nat Med. 2012 Nov;18(11):1682-7. doi: 10.1038/nm.2964. PubMed
Conformation-specific display of 4E10 and 2F5 epitopes on self-assembling protein nanoparticles as a potential HIV vaccine.
Wahome N, Pfeiffer T, Ambiel I, Yang Y, Keppler OT, Bosch V, Burkhard P.
Chem Biol Drug Des. 2012 Sep;80(3):349-57. doi: 10.1111/j.1747-0285.2012.01423.x. Epub 2012 Jul 3. PubMed
A plant-derived flavonoid inhibits entry of all HCV genotypes into human hepatocytes.
Haid S, Novodomská A, Gentzsch J, Grethe C, Geuenich S, Bankwitz D, Chhatwal P, Jannack B, Hennebelle T, Bailleul F, Keppler OT, Poenisch M, Bartenschlager R, Hernandez C, Lemasson M, Rosenberg AR, Wong-Staal F, Davioud-Charvet E, Pietschmann T.
Gastroenterology. 2012 Jul;143(1):213-22.e5. doi: 10.1053/j.gastro.2012.03.036. Epub 2012 Mar 27. PubMed
HIV-1 Vpu's lipid raft association is dispensable for counteraction of the particle release restriction imposed by CD317/Tetherin.
Fritz JV, Tibroni N, Keppler OT, Fackler OT.
Virology. 2012 Mar 1;424(1):33-44. doi: 10.1016/j.virol.2011.12.008. Epub 2012 Jan 4. PubMed
2011
The cellular antiviral restriction factor tetherin does not inhibit poxviral replication.
Sliva K, Resch T, Kraus B, Goffinet C, Keppler OT, Schnierle BS.
J Virol. 2012 Feb;86(3):1893-6. doi: 10.1128/JVI.05198-11. Epub 2011 Nov 16. PubMed
In vivo expression profile of the antiviral restriction factor and tumor-targeting antigen CD317/BST-2/HM1.24/tetherin in humans.
Erikson E, Adam T, Schmidt S, Lehmann-Koch J, Over B, Goffinet C, Harter C, Bekeredjian-Ding I, Sertel S, Lasitschka F, Keppler OT.
Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13688-93. doi: 10.1073https://www.ncbi.nlm.nih.gov/pnas.1101684108. Epub 2011 Aug 1. PubMed
Architecture and regulation of the HIV-1 assembly and holding compartment in macrophages.
Welsch S, Groot F, Kräusslich HG, Keppler OT, Sattentau QJ.
J Virol. 2011 Aug;85(15):7922-7. doi: 10.1128/JVI.00834-11. Epub 2011 May 25. PubMed
HIV-1 Vpu blocks recycling and biosynthetic transport of the intrinsic immunity factor CD317/tetherin to overcome the virion release restriction.
Schmidt S, Fritz JV, Bitzegeio J, Fackler OT, Keppler OT.
MBio. 2011 May 24;2(3):e00036-11. doi: 10.1128/mBio.00036-11. Print 2011. PubMed
β-TrCP is dispensable for Vpu's ability to overcome the CD317/Tetherin-imposed restriction to HIV-1 release. Tervo HM, Homann S, Ambiel I, Fritz JV, Fackler OT, Keppler OT.
Retrovirology. 2011 Feb 10;8:9. doi: 10.1186/1742-4690-8-9. PubMed
2010
High natural permissivity of primary rabbit cells for HIV-1, with a virion infectivity defect in macrophages as the final replication barrier.
Tervo HM, Keppler OT.
J Virol. 2010 Dec;84(23):12300-14. doi: 10.1128/JVI.01607-10. Epub 2010 Sep 22. PubMed
Endogenous CD317/Tetherin limits replication of HIV-1 and murine leukemia virus in rodent cells and is resistant to antagonists from primate viruses.
Goffinet C, Schmidt S, Kern C, Oberbremer L, Keppler OT.
J Virol. 2010 Nov;84(21):11374-84. doi: 10.1128/JVI.01067-10. Epub 2010 Aug 11. PubMed
Poor performance of bioinformatics programs for genotypic prediction of coreceptor usage of HIV-1 group O isolates.
Rupp D, Geuenich S, Keppler OT.
J Acquir Immune Defic Syndr. 2010 Mar;53(3):412-3. doi: 10.1097/QAI.0b013e3181c9f53f. No abstract available. PubMed
Antagonism of CD317 restriction of human immunodeficiency virus type 1 (HIV-1) particle release and depletion of CD317 are separable activities of HIV-1 Vpu.
Goffinet C, Homann S, Ambiel I, Tibroni N, Rupp D, Keppler OT, Fackler OT.
J Virol. 2010 Apr;84(8):4089-94. doi: 10.1128/JVI.01549-09. Epub 2010 Feb 10. PubMed
2009
Biological signature characteristics of primary isolates from human immunodeficiency virus type 1 group O in ex vivo human tonsil histocultures.
Geuenich S, Kaderali L, Allespach I, Sertel S, Keppler OT.
J Virol. 2009 Oct;83(20):10494-503. doi: 10.1128/JVI.00928-09. Epub 2009 Aug 12. PubMed
HIV-1 Gag processing intermediates trans-dominantly interfere with HIV-1 infectivity.
Müller B, Anders M, Akiyama H, Welsch S, Glass B, Nikovics K, Clavel F, Tervo HM, Keppler OT, Kräusslich HG.
J Biol Chem. 2009 Oct 23;284(43):29692-703. doi: 10.1074/jbc.M109.027144. Epub 2009 Aug 7. PubMed
Pharmacovirological impact of an integrase inhibitor on human immunodeficiency virus type 1 cDNA species in vivo.
Goffinet C, Allespach I, Oberbremer L, Golden PL, Foster SA, Johns BA, Weatherhead JG, Novick SJ, Chiswell KE, Garvey EP, Keppler OT.
J Virol. 2009 Aug;83(15):7706-17. doi: 10.1128/JVI.00683-09. Epub 2009 May 20. PubMed
HIV pseudovirion vaccine exposing Env "fusion intermediates"-response to immunisation in human CD4/CCR5-transgenic rats.
Bosch V, Pfeiffer T, Devitt G, Allespach I, Ebensen T, Emerson V, Guzman CA, Keppler OT.
Vaccine. 2009 Apr 6;27(16):2202-12. doi: 10.1016/j.vaccine.2009.02.014. Epub 2009 Feb 13. PubMed
HIV-1 antagonism of CD317 is species specific and involves Vpu-mediated proteasomal degradation of the restriction factor.
Goffinet C, Allespach I, Homann S, Tervo HM, Habermann A, Rupp D, Oberbremer L, Kern C, Tibroni N, Welsch S, Krijnse-Locker J, Banting G, Kräusslich HG, Fackler OT, Keppler OT.
Cell Host Microbe. 2009 Mar 19;5(3):285-97. doi: 10.1016/j.chom.2009.01.009. PubMed
Determinants in HIV-1 Nef for enhancement of virus replication and depletion of CD4+ T lymphocytes in human lymphoid tissue ex vivo.
Homann S, Tibroni N, Baumann I, Sertel S, Keppler OT, Fackler OT.
Retrovirology. 2009 Jan 15;6:6. doi: 10.1186/1742-4690-6-6. PubMed
Human cyclin T1 expression ameliorates a T-cell-specific transcriptional limitation for HIV in transgenic rats, but is not sufficient for a spreading infection of prototypic R5 HIV-1 strains ex vivo.
Michel N, Goffinet C, Ganter K, Allespach I, Kewalramani VN, Saifuddin M, Littman DR, Greene WC, Goldsmith MA, Keppler OT.
Retrovirology. 2009 Jan 13;6:2. doi: 10.1186/1742-4690-6-2. PubMed
2008
Mouse T-cells restrict replication of human immunodeficiency virus at the level of integration.
Tervo HM, Goffinet C, Keppler OT.
Retrovirology. 2008 Jul 8;5:58. doi: 10.1186/1742-4690-5-58. PubMed
High-level transfection of primary rabbit T lymphocytes.
Tervo HM, Allespach I, Keppler OT.
J Immunol Methods. 2008 Jul 20;336(1):85-9. doi: 10.1016/j.jim.2008.03.006. Epub 2008 Apr 7. PubMed
Aqueous extracts from peppermint, sage and lemon balm leaves display potent anti-HIV-1 activity by increasing the virion density.
Geuenich S, Goffinet C, Venzke S, Nolkemper S, Baumann I, Plinkert P, Reichling J, Keppler OT.
Retrovirology. 2008 Mar 20;5:27. doi: 10.1186/1742-4690-5-27. PubMed
2007
Semen-derived amyloid fibrils drastically enhance HIV infection.
Münch J, Rücker E, Ständker L, Adermann K, Goffinet C, Schindler M, Wildum S, Chinnadurai R, Rajan D, Specht A, Giménez-Gallego G, Sánchez PC, Fowler DM, Koulov A, Kelly JW, Mothes W, Grivel JC, Margolis L, Keppler OT, Forssmann WG, Kirchhoff F.
Cell. 2007 Dec 14;131(6):1059-71. PubMed
Primary T-cells from human CD4/CCR5-transgenic rats support all early steps of HIV-1 replication including integration, but display impaired viral gene expression.
Goffinet C, Michel N, Allespach I, Tervo HM, Hermann V, Kräusslich HG, Greene WC, Keppler OT.
Retrovirology. 2007 Jul 26;4:53. PubMed
HIV-1 buds predominantly at the plasma membrane of primary human macrophages.
Welsch S, Keppler OT, Habermann A, Allespach I, Krijnse-Locker J, Kräusslich HG.
PLoS Pathog. 2007 Mar;3(3):e36. PubMed
Analysis of the exposure of induced HIV glycoprotein epitopes in a potential HIV pseudovirion vaccine.
Devitt G, Emerson V, Pisch T, Keppler OT, Bosch V.
Vaccine. 2007 Mar 8;25(12):2161-7. Epub 2006 Dec 27. PubMed
HIV-susceptible transgenic rats allow rapid preclinical testing of antiviral compounds targeting virus entry or reverse transcription.
Goffinet C, Allespach I, Keppler OT.
Proc Natl Acad Sci U S A. 2007 Jan 16;104(3):1015-20. Epub 2007 Jan 5. PubMed
2006
Expression of Nef downregulates CXCR4, the major coreceptor of human immunodeficiency virus, from the surfaces of target cells and thereby enhances resistance to superinfection.
Venzke S, Michel N, Allespach I, Fackler OT, Keppler OT.
J Virol. 2006 Nov;80(22):11141-52. Epub 2006 Aug 23. PubMed
Role of macrophages in HIV infection and persistence.
Venzke S, Keppler OT.
Expert Rev Clin Immunol. 2006 Jul;2(4):613-26. doi: 10.1586/1744666X.2.4.613. PubMed
The Nef protein of human immunodeficiency virus is a broad-spectrum modulator of chemokine receptor cell surface levels that acts independently of classical motifs for receptor endocytosis and Galphai signaling.
Michel N, Ganter K, Venzke S, Bitzegeio J, Fackler OT, Keppler OT.
Mol Biol Cell. 2006 Aug;17(8):3578-90. Epub 2006 Jun 14. PubMed
The HIV-1 pathogenicity factor Nef interferes with maturation of stimulatory T-lymphocyte contacts by modulation of N-Wasp activity.
Haller C, Rauch S, Michel N, Hannemann S, Lehmann MJ, Keppler OT, Fackler OT. J Biol Chem. 2006 Jul 14;281(28):19618-30. Epub 2006 May 10. PubMed
Sensory neuropathy in human immunodeficiency virus/acquired immunodeficiency syndrome patients: protease inhibitor-mediated neurotoxicity.
Pettersen JA, Jones G, Worthington C, Krentz HB, Keppler OT, Hoke A, Gill MJ, Power C.
Ann Neurol. 2006 May;59(5):816-24. PubMed
Efficient nonviral gene delivery into primary lymphocytes from rats and mice.
Goffinet C, Keppler OT.
FASEB J. 2006 Mar;20(3):500-2. Epub 2006 Jan 9. PubMed
2005
Modulation of specific surface receptors and activation sensitization in primary resting CD4+ T lymphocytes by the Nef protein of HIV-1.
Keppler OT, Tibroni N, Venzke S, Rauch S, Fackler OT.
J Leukoc Biol. 2006 Mar;79(3):616-27. Epub 2005 Dec 19. PubMed
The Nef protein of human immunodeficiency virus establishes superinfection immunity by a dual strategy to downregulate cell-surface CCR5 and CD4.
Michel N, Allespach I, Venzke S, Fackler OT, Keppler OT.
Curr Biol. 2005 Apr 26;15(8):714-23. PubMed
Tissue-resident macrophages are productively infected ex vivo by primary X4 isolates of human immunodeficiency virus type 1.
Jayakumar P, Berger I, Autschbach F, Weinstein M, Funke B, Verdin E, Goldsmith MA, Keppler OT.
J Virol. 2005 Apr;79(8):5220-6. PubMed
Peripheral nerve-derived HIV-1 is predominantly CCR5-dependent and causes neuronal degeneration and neuroinflammation.
Jones G, Zhu Y, Silva C, Tsutsui S, Pardo CA, Keppler OT, McArthur JC, Power C.
Virology. 2005 Apr 10;334(2):178-93. PubMed
Rodent cells support key functions of the human immunodeficiency virus type 1 pathogenicity factor Nef.
Keppler OT, Allespach I, Schüller L, Fenard D, Greene WC, Fackler OT.
J Virol. 2005 Feb;79(3):1655-65. PubMed
2004
Construction and characterization of an HIV-1 group O infectious molecular clone and analysis of vpr- and nef-negative derivatives.
Tebit DM, Zekeng L, Kaptué L, Gürtler L, Fackler OT, Keppler OT, Herchenröder O, Kräusslich HG.
Virology. 2004 Sep 1;326(2):329-39. PubMed
2003
In vivo evolution of human immunodeficiency virus type 1 toward increased pathogenicity through CXCR4-mediated killing of uninfected CD4 T cells.
Jekle A, Keppler OT, De Clercq E, Schols D, Weinstein M, Goldsmith MA.
J Virol. 2003 May;77(10):5846-54. PubMed
2002
Progress toward a human CD4/CCR5 transgenic rat model for de novo infection by human immunodeficiency virus type 1.
Keppler OT, Welte FJ, Ngo TA, Chin PS, Patton KS, Tsou CL, Abbey NW, Sharkey ME, Grant RM, You Y, Scarborough JD, Ellmeier W, Littman DR, Stevenson M, Charo IF, Herndier BG, Speck RF, Goldsmith MA.
J Exp Med. 2002 Mar 18;195(6):719-36. PubMed
2001
Versatile biosynthetic engineering of sialic acid in living cells using synthetic sialic acid analogues.
Oetke C, Brossmer R, Mantey LR, Hinderlich S, Isecke R, Reutter W, Keppler OT, Pawlita M.
J Biol Chem. 2002 Feb 22;277(8):6688-95. Epub 2001 Dec 20. PubMed
2000 and before
Susceptibility of rat-derived cells to replication by human immunodeficiency virus type 1.
Keppler OT, Yonemoto W, Welte FJ, Patton KS, Iacovides D, Atchison RE, Ngo T, Hirschberg DL, Speck RF, Goldsmith MA.
J Virol. 2001 Sep;75(17):8063-73. PubMed
Efficient biochemical engineering of cellular sialic acids using an unphysiological sialic acid precursor in cells lacking UDP-N-acetylglucosamine 2-epimerase.
Mantey LR, Keppler OT, Pawlita M, Reutter W, Hinderlich S.
FEBS Lett. 2001 Aug 10;503(1):80-4. PubMed
Evidence for efficient uptake and incorporation of sialic acid by eukaryotic cells.
Oetke C, Hinderlich S, Brossmer R, Reutter W, Pawlita M, Keppler OT.
Eur J Biochem. 2001 Aug;268(16):4553-61. PubMed
Biosynthesis of N-acetylneuraminic acid in cells lacking UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase.
Hinderlich S, Berger M, Keppler OT, Pawlita M, Reutter W.
Biol Chem. 2001 Feb;382(2):291-7. PubMed
Biochemical engineering of the N-acyl side chain of sialic acid: biological implications. Keppler OT, Horstkorte R, Pawlita M, Schmidt C, Reutter W. Glycobiology. 2001 Feb;11(2):11R-18R. Review. PubMed
Differential sialylation of cell surface glycoconjugates in a human B lymphoma cell line regulates susceptibility for CD95 (APO-1/Fas)-mediated apoptosis and for infection by a lymphotropic virus.
Keppler OT, Peter ME, Hinderlich S, Moldenhauer G, Stehling P, Schmitz I, Schwartz-Albiez R, Reutter W, Pawlita M.
Glycobiology. 1999 Jun;9(6):557-69. PubMed
UDP-GlcNAc 2-epimerase: a regulator of cell surface sialylation.
Keppler OT, Hinderlich S, Langner J, Schwartz-Albiez R, Reutter W, Pawlita M.
Science. 1999 May 21;284(5418):1372-6. PubMed
Elongation of the N-acyl side chain of sialic acids in MDCK II cells inhibits influenza A virus infection.
Keppler OT, Herrmann M, von der Lieth CW, Stehling P, Reutter W, Pawlita M.
Biochem Biophys Res Commun. 1998 Dec 18;253(2):437-42. PubMed
Biosynthetic modulation of sialic acid-dependent virus-receptor interactions of two primate polyoma viruses.
Keppler OT, Stehling P, Herrmann M, Kayser H, Grunow D, Reutter W, Pawlita M.
J Biol Chem. 1995 Jan 20;270(3):1308-14. PubMed
Regulation of susceptibility and cell surface receptor for the B-lymphotropic papovavirus by N glycosylation.
Keppler OT, Herrmann M, Oppenländer M, Meschede W, Pawlita M.
J Virol. 1994 Nov;68(11):6933-9. PubMed
The cell surface receptor is a major determinant restricting the host range of the B-lymphotropic papovavirus.
Haun G, Keppler OT, Bock CT, Herrmann M, Zentgraf H, Pawlita M.
J Virol. 1993 Dec;67(12):7482-92. PubMed
Human Golgi beta-galactoside alpha-2,6-sialyltransferase generates a group of sialylated B lymphocyte differentiation antigens.
Keppler OT, Moldenhauer G, Oppenländer M, Schwartz-Albiez R, Berger EG, Funderud S, Pawlita M.
Eur J Immunol. 1992 Nov;22(11):2777-81. PubMed