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Keppler Lab - Research

Studies on HIV Pathogenesis in ex vivo Tissue Histocultures

The human lymphoid ex vivo histoculture model, derived from routine tonsillectomy material, is a biologically relevant experimental platform permissive for HIV infection independent of exogenous stimulation by mitogens or interleukin-2. This histoculture maintains the tonsil’s natural cytokine milieu, cellular activation status, and cell-to-cell interactions.

In this valuable ex vivo model system to address the following questions

  • Define differences in replication potential and pathogenesis of primary, patient-derived HIV-1 isolates with respect to their variable coreceptor usage, clinical behavior, and contribution to the HIV pandemic
  • Explore mechanisms of HIV-induced cell death