Bohlen Lab - mRNA Translation in Human Immunity
Research Topics
- Translational Regulation in T Cells
- Monogenic Ribosomopathies and Immunodeficiency
- tRNA Metabolism, Biogenesis, and Autoinflammatory Disease
Research Summary
Our research group is dedicated to investigating the regulation of mRNA translation and its critical role in cellular and organismal processes. Despite its importance, translational control in human physiology remains poorly understood, primarily due to the limited availability of live, primary samples at scale. Leukocyte biology offers a practical model for studying mRNA translation in primary physiological functions. Human leukocytes are readily available and can be cultured in large quantities, making them an ideal system for our research. Read more...
Join Our Team
We aim to achieve three significant outcomes:
1. Enhanced understanding of ribosome and mRNA biology in leukocytes.
2. Insights into leukocyte involvement in genetic diseases of mRNA translation.
3. Identification of pathomechanisms underlying severe and untreatable genetic diseases.
We welcome students interested in contributing to this research. Join us to explore the genetic foundations of human health and help bridge basic science with clinical insights. For more information and to get involved, please contact us.
Selected Publications
Autoinflammation in patients with leukocytic CBL loss of heterozygosity is caused by constitutive ERK-mediated monocyte activation
Bohlen J, Bagarić I, Vatovec T, Ogishi M, Ahmed SF, Cederholm A, Buetow L, Sobrino S, Le Floc'h C, Arango-Franco CA, Seabra L, Michelet M, Barzaghi F, Leardini D, Saettini F, Vendemini F, Baccelli F, Catala A, Gambineri E, Veltroni M, Aguilar de la Red Y, Rice GI, Consonni F, Berteloot L, Largeaud L, Conti F, Roullion C, Masson C, Bessot B, Seeleuthner Y, Le Voyer T, Rinchai D, Rosain J, Neehus AL, Erazo-Borrás L, Li H, Janda Z, Cho EJ, Muratore E, Soudée C, Lainé C, Delabesse E, Goulvestre C, Ma CS, Puel A, Tangye SG, André I, Bole-Feysot C, Abel L, Erlacher M, Zhang SY, Béziat V, Lagresle-Peyrou C, Six E, Pasquet M, Alsina L, Aiuti A, Zhang P, Crow YJ, Landegren N, Masetti R, Huang DT, Casanova JL, Bustamante J.
J Clin Invest. 2024 Oct 15;134(20):e181604. doi: 10.1172/JCI181604. PubMed
Human MCTS1-dependent translation of JAK2 is essential for IFN-γ immunity to mycobacteria.
Bohlen J, Zhou Q, Philippot Q, Ogishi M, Rinchai D, Nieminen T, Seyedpour S, Parvaneh N, Rezaei N, Yazdanpanah N, Momenilandi M, Conil C, Neehus AL, Schmidt C, Arango-Franco CA, Voyer TL, Khan T, Yang R, Puchan J, Erazo L, Roiuk M, Vatovec T, Janda Z, Bagarić I, Materna M, Gervais A, Li H, Rosain J, Peel JN, Seeleuthner Y, Han JE, L'Honneur AS, Moncada-Vélez M, Martin-Fernandez M, Horesh ME, Kochetkov T, Schmidt M, AlShehri MA, Salo E, Saxen H, ElGhazali G, Yatim A, Soudée C, Sallusto F, Ensser A, Marr N, Zhang P, Bogunovic D, Cobat A, Shahrooei M, Béziat V, Abel L, Wang X, Boisson-Dupuis S, Teleman AA, Bustamante J, Zhang Q, Casanova JL.
Cell. 2023 Nov 9;186(23):5114-5134.e27. PubMed
PRRC2 proteins impact translation initiation by promoting leaky scanning.
Bohlen J, Roiuk M, Neff M, Teleman AA.
Nucleic Acids Res. 2023 Apr 24;51(7):3391-3409. PubMed
Selective 40S Footprinting Reveals Cap-Tethered Ribosome Scanning in Human Cells.
Bohlen J, Fenzl K, Kramer G, Bukau B, Teleman AA.
Mol Cell. 2020 Aug 20;79(4):561-574.e5. PubMed