Stigler Lab - Biophysics of Structural Dynamics in Chromosomes
Research Topics
- Single Molecule Biophysics
- Protein Folding Mechanics
- Mechanics of Intrachromosomal Crosslinks
- Establishment of Hierarchically Structured Chromosomes
Prof. Johannes Stigler
phone: +49 (0)89 - 2180 76951
stigler@genzentrum.lmu.de
Chromosomes provide the fundamental scaffold for the maintenance, regulation and propagation of genetic information. Recent experimental findings have shown that eukaryotic chromosomes are highly structured and that the three-dimensional folding of chromosomes is directly linked to gene regulation. Our lab uses biophysical single molecule techniques to determine the processes that lead to the three-dimensional folding of chromosomes. Read more...
Selected Publications
Single-molecule imaging reveals a direct role of CTCF’s zinc fingers in SA interaction and cluster-dependent RNA recruitment.
Huber J, Tanasie N-L, Zernia S, Stigler J.
Nucleic Acids Research. 2024; 52(11):6490-6506. doi: 10.1093/nar/gkae391
ISWI catalyzes nucleosome sliding in condensed nucleosome arrays.
Vizjak P, Kamp D, Hepp N, Scacchetti A, Gonzalez Pisfil M, Bartho J, Halic M, Becker PB, Smolle M, Stigler J*, Mueller-Planitz F*.
Nature Structural & Molecular Biology. 2024; 31(19):1331-1340. doi: 10.1038/s41594-024-01290-x
Stabilization of DNA fork junctions by Smc5/6 complexes revealed by single-molecule imaging.
Tanasie N-L, Gutiérrez-Escribano P, Jaklin S, Aragon L, Stigler J.
Cell Reports. 2022; 41(10):111778. doi: 10.1016/j.celrep.2022.111778
Unraveling the Mechanics of a Repeat-Protein Nanospring: From Folding of Individual Repeats to Fluctuations of the Superhelix.
Synakewicz M, Eapen RS, Perez-Riba A, Rowling PJE, Bauer D, Weißl A, Fischer G, Hyvönen M, Rief M, Itzhaki LS, Stigler J.
ACS Nano. 2022; 16(3):3895-3905. doi: 10.1021/acsnano.1c09162
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