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Stigler Lab - Biophysics of Structural Dynamics in Chromosomes

stigler_mainResearch Topics

  • Mechanics of Intrachromosomal Crosslinks
  • Establishment of Hierarchically Structured Chromosomes

Prof. Johannes Stigler

phone: +49 (0)89 - 2180 76951

Chromosomes provide the fundamental scaffold for the maintenance, regulation and propagation of genetic information. Recent experimental findings have shown that eukaryotic chromosomes are highly structured and that the three-dimensional folding of chromosomes is directly linked to gene regulation. Our lab uses biophysical single molecule techniques to determine the processes that lead to the three-dimensional folding of chromosomes. Read more...

Selected Publications

Single-Molecule Imaging Reveals a Collapsed Conformational State for DNA-Bound Cohesin.
Stigler J, Çamdere GÖ, Koshland DE, Greene EC.
Cell Rep. 2016 May 3;15(5):988-998. doi: 10.1016/j.celrep.2016.04.003. Epub 2016 Apr 21. PubMed

Sequence-resolved free energy profiles of stress-bearing vimentin intermediate filaments.
Ramm B, Stigler J, Hinczewski M, Thirumalai D, Herrmann H, Woehlke G, Rief M.
Proc Natl Acad Sci U S A. 2014 Aug 5;111(31):11359-64. doi: 10.1073/pnas.1403122111. Epub 2014 Jul 21. PubMed

The complex folding network of single calmodulin molecules.
Stigler J, Ziegler F, Gieseke A, Gebhardt JC, Rief M.
Science. 2011 Oct 28;334(6055):512-6. doi: 10.1126/science.1207598. PubMed

More publications


New publication

Sarah describes in her methods book chapter how DNA curtains can be used to study co-condensation of proteins with DNA.

New publication

Loredana's new paper shows that Smc5/6 holocomplexes stabilize forks at sites of DNA lesion.

New member in our lab

In October 2022 we welcome our new technician Joelle Deplazes-Lauber!

New publication

Our study of the folding of consensus TPR proteins, together with Marie Synakewicz, Laura Itzhaki and Matthias Rief, was published in ACS Nano.