Stingele Lab - Cellular Biochemistry
Research Topics
- Nucleic acid quality control
- Repair of DNA-protein crosslinks
- Resolution of RNA damage
Nucleic acid quality control is essential for cellular homeostastis and to counteract cancer development and ageing. We are trying to understand how cells detect and resolve damage to DNA and RNA. We investigate these processes by combining various approaches including cell biology, genetic engineering, proteomics, and in vitro reconstitution. Read more...
Selected Publications
Transcription-coupled repair of DNA-protein cross-links depends on CSA and CSB.
Carnie CJ, Acampora AC, Bader AS, Erdenebat C, Zhao S, Bitensky E, van den Heuvel D, Parnas A, Gupta V, D'Alessandro G, Sczaniecka-Clift M, Weickert P, Aygenli F, Götz MJ, Cordes J, Esain-Garcia I, Melidis L, Wondergem AP, Lam S, Robles MS, Balasubramanian S, Adar S, Luijsterburg MS, Jackson SP, Stingele J.
Nat Cell Biol. 2024 May;26(5):797-810. doi: 10.1038/s41556-024-01391-1. Epub 2024 Apr 10. PubMed
RNF14-dependent atypical ubiquitylation promotes translation-coupled resolution of RNA-protein crosslinks.
Zhao S, Cordes J, Caban KM, Götz MJ, Mackens-Kiani T, Veltri AJ, Sinha NK, Weickert P, Kaya S, Hewitt G, Nedialkova DD, Fröhlich T, Beckmann R, Buskirk AR, Green R, Stingele J.
Mol Cell. 2023 Nov 2:S1097-2765(23)00849-3. doi: 10.1016/j.molcel.2023.10.012. PubMed
The FANCJ helicase unfolds DNA-protein crosslinks to promote their repair.
Yaneva D, Sparks JL, Donsbach M, Zhao S, Weickert P, Bezalel-Buch R, Stingele J*, Walter JC*.
Mol Cell. 2023 Jan 5;83(1):43-56.e10. doi: 10.1016/j.molcel.2022.12.005. PubMed
DNA Structure-Specific Cleavage of DNA-Protein Crosslinks by the SPRTN Protease.
Reinking HK, Kang HS, Götz MJ, Li HY, Kieser A, Zhao S, Acampora AC, Weickert P, Fessler E, Jae LT, Sattler M, Stingele J.
Mol Cell. 2020 Oct 1;80(1):102-113.e6. doi: 10.1016/j.molcel.2020.08.003. Epub 2020 Aug 26. PubMed
More publications
News
November 2024 - New Preprint
Allosteric activation of the SPRTN protease by ubiquitin maintains genome stability
November 2024 - New Review
New review on the transcription-coupled repair of DNA–protein crosslinks