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Arulanandam (JP) Lab - Structural Biology of Cell Division

jp_mainResearch Topics

  • Integrative Structural Cell Biology
  • Mechanisms of Chromosome Segregation
  • RNA Modifications and Spindle Assembly

Prof. Jeyaprakash Arulanandam (JP)

His ERC-funded lab has started at the Gene Center on 1st March 2023.

phone: +49 (0)89 - 2180 76962
jparul@genzentrum.lmu.de

We are interested in understanding the mechanism of chromosome segregation during cell division. Particularly, we aim to obtain high-resolution mechanistic insights into how centromere assembly and function and RNA-modification activities ensure error-free chromosome segregation. Towards this goal, we employ an integrative structure-function approach combining cryoEM, Crystallography and CrossLinking/MS with proteomics and cell biology.

Selected Publications

PLK1-mediated phosphorylation cascade activates Mis18 complex to ensure centromere inheritance.
Parashara P*, Medina-Pritchard B*, Abad MA*, Sotelo-Parrilla P*, Thamkachy R, Grundei D, Zou J, Spanos C, Kumar CN, Basquin C, Das V, Yan Z, Al-Murtadha AA, Kelly DA, McHugh T, Imhof A, Rappsilber J, Jeyaprakash AA.
Science. 2024 Sep 6;385(6713):1098-1104. doi: 10.1126/science.ado8270. (*equal contribution) PubMed

Structural basis for Mis18 complex assembly and its implications for centromere maintenance.
Thamkachy R*, Medina-Pritchard B*, Park SH*, Chiodi CG, Zou J, de la Torre-Barranco M, Shimanaka K, Abad MA, Gallego Páramo C, Feederle R, Ruksenaite E, Heun P, Davies OR, Rappsilber J, Schneidman-Duhovny D, Cho US, Jeyaprakash AA.
EMBO Rep. 2024 Aug;25(8):3348-3372. doi: 10.1038/s44319-024-00183-w. (*equal contribution) PubMed

Mechanistic Basis for Sgo1-Mediated Centromere Localisation and Function of the CPC.
Abad MA*, Gupta T*, Hadders MA, Meppelink A, Wopken JP, Blackburn E, Zou J, Gireesh A, Buzuk L, Kelly DA, McHugh T, Rappsilber J, Lens SMA, Jeyaprakash AA
J. Cell Biol (2022) 221(8):e202108156 Doi:10.1083/jcb.202108156 (*equal contribution) PubMed

Structural Basis for Centromere Maintenance by Drosophila CENP-A Chaperone Cal1.
Medina-Pritchard B, Lazou V, Zou J, Byron O, Abad MA, Rappsilber J, Heun P, Jeyaprakash AA
EMBO J (2020) Doi:10.15252/embj.2019103234. PubMed

 

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