Adler Lab - Research
One central tool to study the role of viral proteins are cytomegalovirus genomes cloned as bacterial artificial chromosomes (BAC). Cloning herpesvirus genomes as BAC involves insertion of huge (200.000 bp) long DNA genomes in bacterial vectors, subsequent mutagenesis in E.coli and reconstitution of these modified genomes by transfection of host cells. Using BAC technology, we can mutate, delete, or overexpress viral proteins or insert reporter genes like GFP or luciferase.
Figure: Cloning of large virus genomes as bacterial artificial chromosomes –
a technique to produce state of the art virus mutants