Fröhlich Lab - Proteomics
- Mammalian Embryo and Germ Cell Proteomics
- Proteomics of Animal Disease Models
- Proteomic Approach to Testicular Ageing
Our research activity comprises proteome analysis during early embryogenesis in mammals, proteomic characterization of large animal disease models for molecular biomedical research (Diabetes, Duchenne muscular dystrophy), and secretome analysis of human testicular peritubular cells (HTPC) in the context of spermatogenesis. We perform holistic as well as targeted proteomic approaches using latest mass spectrometry instrumentation. Read more...
Progressive muscle proteome changes in a clinically relevant pig model of Duchenne muscular dystrophy.
Fröhlich T, Kemter E, Flenkenthaler F, Klymiuk N, Otte KA, Blutke A, Krause S, Walter MC, Wanke R, Wolf E, Arnold GJ.
Sci Rep. 2016 Sep 16;6:33362. doi: 10.1038/srep33362. PubMed
Proteome analysis of early lineage specification in bovine embryos.
Demant M, Deutsch DR, Fröhlich T, Wolf E, Arnold GJ.
Proteomics. 2015 Feb;15(4):688-701. doi: 10.1002. Epub 2014 Oct 9. PubMed
Stage-specific proteome signatures in early bovine embryo development.
Deutsch DR, Fröhlich T, Otte KA, Beck A, Habermann FA, Wolf E, Arnold GJ.
J Proteome Res. 2014 Oct 3;13(10):4363-76. doi: 10.1021. Epub 2014 Sep 10. PubMed
Secretome analysis of testicular peritubular cells: a window into the human testicular microenvironment and the spermatogonial stem cell niche in man.
Flenkenthaler F, Windschüttl S, Fröhlich T, Schwarzer JU, Mayerhofer A, Arnold GJ.
J Proteome Res. 2014 Mar 7;13(3):1259-69. doi: 10.1021. Epub 2014 Jan 30. PubMed
1.1.2018: New QExactive HF-X mass spectrometer
A new QExactive HF-X mass spectrometer was installed at the LAFUGA proteomics facility and is now fully operational. The recently released QExactive HF-X is a state-of-the-art fast scanning high resolution mass spectrometer, allowing the in-depth proteome analysis of complex protein mixtures. Compared to our previous instruments, the sensitivity as well as the peptide identification rate is significantly improved.